나노생명공학 연구실에서는 질병의 조기진단 및 환경 문제 해결에 기여할 수 있는 나노 생명 공학 분야 기술들을 여러 가지 측면에서 접근하여 연구를 수행하고 있습니다. 본 연구진은 금 나노입자와 폴리디아세틸렌 고분자를 사용함으로써 비표지 물질로 분자영상기술 구현을 가능하게 하여 실용화가 가능한 차세대 나노 바이오센서 시스템을 구축하고. 보유하고 있는 초고감도 검출이 가능한 LSPR용 암시야 현미경 시스템과 표면 개질 기술, 다양한 리셉터의 활용 기술, 신호 증폭을 통한 이미지 성능 개선 등을 통하여 다양한 질병 모델에 적용할 수 있는 시스템의 개발을 적극적으로 추진하고 있습니다.
At the Nanobiotechnology lab we are conducting research on various nano biotechnology techniques focused on early diagnosis and environmental issues. The research team is currently promoting various projects, which can be applied to various disease models. Such projects include utilizing inorganic substances such as gold nanoparticles and polyacetylene to actualize molecular imaging technology; developing a next generation nano biosensor system, which can be commercialized; developing LSPR darkfield microscope with a supersensitive detection technology along with surface reforming technology; applying various receptor technologies; and enhancing imagery using an amplifier.

나노생명공학 연구실에서는 질병의 조기진단 및 환경 문제 해결에 기여할 수 있는 나노 생명 공학 분야 기술들을 여러 가지 측면에서 접근하여 연구를 수행하고 있습니다. 본 연구진은 금 나노입자와 폴리디아세틸렌 고분자를 사용함으로써 비표지 물질로 분자영상기술 구현을 가능하게 하여 실용화가 가능한 차세대 나노 바이오센서 시스템을 구축하고. 보유하고 있는 초고감도 검출이 가능한 LSPR용 암시야 현미경 시스템과 표면 개질 기술, 다양한 리셉터의 활용 기술, 신호 증폭을 통한 이미지 성능 개선 등을 통하여 다양한 질병 모델에 적용할 수 있는 시스템의 개발을 적극적으로 추진하고 있습니다.

Spectroscopic Analysis of Live Cancer Cell Microenvironment with Terahertz Metasurface Biosensing Platform

2P-350 Continuous magnetic separation of microdroplets having different microalgal cell densities in microfluidic device

2P-347 MALDI-based quantitative targeted metabolomics for 15d-deoxy-Δ12, 14PGJ2 (15d-PGJ2)

Dual stimuli-responsive smart fluid of spiropyran-coated silica nanoparticles

2P-345 Development of Wave-powered Pump for Supplying Nutrients to Floating Photobioreactors with Selectively-permeable Membranes

Oral Presentation: 2LF-2; Methanotroph-catalyzed bioconversion of methane to methanol

Oral Presentation: 2LE-10; Conjugated polymer composites for electrodes and energy conversion devices

Oral Presentation: 2LE-9; Electrochemical analysis in lithium-sulfur batteries using carbonaceous materials

2P-159; Silvering of poly (ethylene terephthalate) fibers via in situ thermal reduction of silver (I) carbamate complexes

Oral Presentation: 2LE-8; Characterization of Pt/C catalysts for oxygen reduction reaction using electrochemical methods

2P-156; Synthesis and modification of zirconia with silane coupling agent for produce optical films with high refractive index and high transparency

2P-160; Biosensor of micro arrayed polydiacetylene (PDA) liposome using gold nanoparticles for signal enhancement

2P-383 Molecular Dynamics Simulation Study of Potential Energy for Cellobiose and 1, 1-dimethoxyethane

2P-380 Quantitation of the Chemotactic Response of a Green Microalga, Chlamydomonas reinhardtii, to Bicarbonate Using a Novel Microfluidic Device

2P-157; Highly fluorescent graphene oxide biosensors for determination of AChE activity involved in alzheimer``s disease

2P-385 Engineering aldehyde dehydrogenase based on a synthetic selection device to optimize the 3-hydroxypropionic acid production pathway

2P-384 Development of naringenin riboswitches for sensing and monitoring in vivo

2P-161; 3-D graphene-carbon nanotube-nickel nano-architectures for supercapacitors

2P-209: Wearable charge-trap flash memory electronic devices based on carbon nanotube

2P-202; Solution-deposited hybrid gate dielectric materials

2P-182 내분비계 교란물질을 검출하기 위한 M-13 박테리오파지 기반 구조색 센서

2P-274; Synthesis of barium stannate nanowires for CO₂ gas detecting

2P-272; Directed synthesis of nanoarchitectures by single DNA molecules

2P-374 Enhanced myelination of pluripotent stem cell-derived oligodendrocytes on extracellular matrix-modified nanofibrous scaffolds

2P-174 Nanoplasmonic system of RNA folding and assembly while pre-mRNA splicing is done

2P-170 Colorimetric Detecting Property to Cadmium Ions (Cd2+) depending on Shape of Nano Gold

Improvement in autotrophic production of hydrogen under sulfur deprivation using CO₂ concentrating mechanism (CCM)-induced Chlamydomonas cells

2P-173 Nano carbon material reinforced non-woven carbon fiber based film for enhanced electromagnetic shielding effectiveness

Acidic cultivation of Haematococcus pluvialis to prevent contamination of a lethal fungus, Paraphysoderma sedebokerensis, with improved astaxanthin …

Oral Presentation: 1LH-2; Biological carbon capture and utilization system using enzymes from microalgae

광배양 시스템에서 NOx 및 SOx 의 배지 pH 와미세조류 생장에 미치는 영향

177Lu-Labeled Cyclic-Cholecystokinin Derivative for Peptide-Receptor Radionuclide Therapy

Oral Presentation: 2LC-13; Synthesis and electroluminscence properties of organic emitters including electron donor and acceptor in a molecule

Enhancement of Biomass and Lipid Production via Biomineralization at Mildly Alkaline pH Level in the Thermotolerant Microalgae Chlorella sorokiniana and …

Bio-conjugated nanoarchitectonics with dual-labeled nanoparticles for a colorimetric and fluorescent dual-mode serological lateral flow immunoassay sensor in …

Oral Presentation: 2LC-15; Investigation of the sensitive enhancement in surface plasmon resonance coupling sensor mediated by stimuli-sensitive polymer …

A label-free, ultra-highly sensitive and multiplexed SERS nanoplasmonic biosensor for miRNA detection using a head-flocked gold nanopillar

Photothermal Cancer Therapy Using pH-Sensitive Gold Nanoparticles and Tumor-Tropic Mesenchymal Stem Cells

P0018 Enhanced astaxanthin extraction efficiency and astaxanthin stabliity from Haematococcus pluvialis using biological and chemical treatment

광배양 시스템에서 NOx 및 SOx 의 배지 pH 와 미세조류 생장에 미치는 영향

Microdroplet photobioreactor for the photoautotrophic culture of microalgal cells

2P-287; A study on anti-glare glass with nanostructured surface prepared by the polymer blend lithography and dry etch process

2P-306; Photoautotrophic growth of microalgal cells in microdroplet system

2P-155; EMI Shielding Properties of electrospun carbon fiber web

Ultrasensitive detection of the reduced form of nicotinamide adenine dinucleotide based on carbon nanotube field effect transistor

Novel microfluidic selection of superior strains with high photosynthetic efficiency using competitive phototactic response

Autotrophic Biodiesel Production from the Thermotolerant Microalga Chlorella sorokiniana by Enhancing the Carbon Availability with Temperature Adjustment

Mass transfer properties in modular polymeric thin-film photobioreactor for autotrophically cultivating Haematococcus pluvialis using induistrial flue gas

Femtomolar detection of single mismatches by discriminant analysis of DNA hybridization events using gold nanoparticles

Oral Presentation: 2LC-12; Fabrication of high performance ultra filtration hollwo fiber membrane for water purifier

Plasmonic coupling-dependent SERS of gold nanoparticles anchored on methylated DNA and detection of global DNA methylation in SERS-based platforms

Signal enhancement of a micro-arrayed polydiacetylene (PDA) biosensor using gold nanoparticles

Nanoplasmonic probes of RNA folding and assembly during pre-mRNA splicing

Corrigendum to" Engineering interventions in enzyme production: Lab to industrial scale"

PSP25 Novel microfluidic selection of superior strains with high photosynthetic efficiency using competitive phototactic response

Rational aspect ratio and suitable antibody coverage of gold nanorod for ultra-sensitive detection of a cancer biomarker

Single plasmonic nanostructures for biomedical diagnosis

Enhancement of Microalgal Biomass Productivity by Using Expandable Transparent Film Bag Photobioreactor and Real-time Monitoring System

2P-283; Fabrication of prussian blue nanoparticles coated 3D graphene foam for {}^{137} Cs adsorption from contaminated water

Magnetophoretic Screening Platform for Selection of Fast-growing Microalgal Strains

우수논문 (구두): 2LC-14; 연신 및 친수화 공정 조건 변화에 따른 PVDF 중공사막의 성능 향상

P0152 Mass transfer properties in modular polymeric thin-film photobioreactor for autotrophic cultivation of Haematococcus pluvialis using industrial flue gas

생물공학: 2LF-1; 미세조류의 광합성 효율 향상을 위한 주광성/주화성 기반 고속 스크리닝 전략

미세조류에 의한 이산화탄소의 생물학적 유기자원화

Improvement of Lipid Production with Synergetic Effect of Multiple Stresses in Microalgae

Corrigendum to" Development of SERS substrate using phage-based magnetic template for triplex assay in sepsis diagnosis"[Biosens. Bioelectron. 85 (2016) 522 …

2P-276: Kapok 을 template 로 이용하여 Porous 유-무기 하이브리드 구조체 합성

Científicos Emprendedores

The chemotaxis towards bicarbonate is related to the carbon dioxide utilization, in microalga Chlamydomonas reinhardtii

석유화학, 윤활유: 1LG-6; 지속생산 가능한 트리글리세라이드를 기유로 한 유압 작동유 제조기술

Relation of carbon dioxide utilization and chemotaxis of a microalga, Chlamydomonas reinhardtii, towards bicarbonate

Quantitation of the chemotaxis of Chlamydomonas reinhardtii towards bicarbonate with agarose-gel-based microsystem

Microdroplet based photoautotrophic cultivation of microalgae using poly (dimethylsiloxane)(PDMS) microfluidic device

Split Mixotrophy: A Novel Cultivation Strategy to Enhance Biomass and Lipid Yields of Chlorella protothecoides in Mixotrophic Condition

Professor Sang Jun Sim (沈相俊)

특별구두세션 (KCRC): 1LH-1; 유용물질 자원화를 위한 이산화탄소의 생물학적 이용

Effect of Light Conditions and Mixotrophic Cultivation in Green Microalgae

Peptide-Guided 3D Patterning of Gold Nanoparticles for High-Precision Biosensor Applications

Label-free multi-detection of cancer biomarkers using nanoplasmonic biosensor

Microdroplet-based Screening of Highly Productive Microalgal Strains Under Fluctuating Light Boosts Outdoor Mass Production of Microalgae

The Enhancement of Algal Biofuel Production via Genetic Transformation

P1029 Label-free multi-detection of cancer biomarkers using nanoplasmonic biosensor

Ecotoxicity of Silver Nanoparticles on the Soil Nematode Caenorhabditis elegans Using Functional Ecotoxicogenomics

Enzymatic pretreatment of Chlamydomonas reinhardtii biomass for ethanol production

An in vitro study of the antifungal effect of silver nanoparticles on oak wilt pathogen Raffaelea sp

DNA-free two-gene knockout in Chlamydomonas reinhardtii via CRISPR-Cas9 ribonucleoproteins

Pretreatment of rice straw with ammonia and ionic liquid for lignocellulose conversion to fermentable sugars

The hazardous threat of Bisphenol A: Toxicity, detection and remediation

Hydrocarbon production from secondarily treated piggery wastewater by the green alga Botryococcus braunii

Hydrothermal acid pretreatment of Chlamydomonas reinhardtii biomass for ethanol production

PHA synthase activity controls the molecular weight and polydispersity of polyhydroxybutyrate in vivo

Quantitative and specific detection of exosomal miRNAs for accurate diagnosis of breast cancer using a surface‐enhanced Raman scattering sensor based on plasmonic head‐flocked …

Hydrogen production from Chlamydomonas reinhardtii biomass using a two-step conversion process: anaerobic conversion and photosynthetic fermentation

Effect of light conditions on mixotrophic cultivation of green microalgae

Ultrasensitive carbon nanotube-based biosensors using antibody-binding fragments

Characterization of a self-assembled monolayer of thiol on a gold surface and the fabrication of a biosensor chip based on surface plasmon resonance for detecting anti-GAD antibody

Emerging prospects of mixotrophic microalgae: Way forward to sustainable bioprocess for environmental remediation and cost-effective biofuels

Comparison of heterotrophic and photoautotrophic induction on astaxanthin production by Haematococcus pluvialis

Good things come in small packages: Overcoming challenges to harness extracellular vesicles for therapeutic delivery

Surface-initiated, atom transfer radical polymerization of oligo (ethylene glycol) methyl ether methacrylate and subsequent click chemistry for bioconjugation

A strategy for sensitivity and specificity enhancements in prostate specific antigen-α1-antichymotrypsin detection based on surface plasmon resonance

Enhancement of sensitivity and specificity by surface modification of carbon nanotubes in diagnosis of prostate cancer based on carbon nanotube field effect transistors

Sang Jun Sim

In the present study, the ecotoxicity of silver nanoparticles (AgNPs) was investigated in Caenorhabditis elegans using survival, growth, and reproduction, as the ecotoxicological endpoints, as well as stress response gene expression. Whole genome microarray was used to screen global changes in C. elegans transcription profiles after AgNPs exposure, followed by quantitative analysis of selected genes. The integration of gene expression with organism and population level endpoints was investigated using C. elegans functional genomics tools, to test the ecotoxicological relevance of AgNPs-induced gene expression. AgNPs exerted considerable toxicity in C. elegans, most clearly as dramatically decreased reproduction potential. Increased expression of the superoxide dismutases-3 ( sod-3) and abnormal dauer formation protein ( daf-12 ) genes with 0.1 and 0.5 mg/L of AgNPs exposures occurred concurrently …

The production of ethanol from feedstock other than agriculture materials has been promoted in recent years. Some microalgae can accumulate a high starch content (about 44% of dry base) via photosynthesis. Algal biomass, Chlamydomonas reinhardtii UTEX 90, was converted into a suitable fermentable feedstock by two commercial hydrolytic enzymes. The results showed that almost all starch was released and converted into glucose without steps for the cell wall disruption. Various conditions in the liquefaction and saccharification processes, such as enzyme concentration, pH, temperature, and residence time, have been investigated to obtain an optimum combination using the orthogonal analysis. As a result, approximately 235mg of ethanol was produced from 1.0g of algal biomass by a separate hydrolysis and fermentation (SHF) method. The main advantages of this process include the low cost of …

In this study, we investigated the antifungal activity of three different forms of silver nanoparticles against the unidentified ambrosia fungus Raffaelea sp., which has been responsible for the mortality of a large number of oak trees in Korea. Growth of fungi in the presence of silver nanoparticles was significantly inhibited in a dosedependent manner. We also assessed the effectiveness of combining the different forms of nanoparticles. Microscopic observation revealed that silver nanoparticles caused detrimental effects not only on fungal hyphae but also on conidial germination.

Microalgae are versatile organisms capable of converting CO 2 , H 2 O and sunlight into fuel and chemicals for domestic and industrial consumption. Thus, genetic modifications of microalgae for enhancing photosynthetic productivity and biomass and bio-products generation are crucial for both academic and industrial applications. However, targeted mutagenesis in microalgae with CRISPR-Cas9 is limited. Here we report, a one-step transformation of Chlamydomonas reinhardtii by the DNA-free CRISPR-Cas9 method rather than plasmids that encode Cas9 and guide RNAs. Outcome was the sequential CpFTSY and ZEP two-gene knockout and the generation of a strain constitutively producing zeaxanthin and showing improved photosynthetic productivity.

Pretreatment technology is a prerequisite to facilitate the release of sugars from a lignocellulosic biomass prior to fermentation. Recently, some pretreatment methods have been tried with ionic liquids, but they were still expensive and unpractical. In this study, an efficient pretreatment method using ammonia and ionic liquid was developed for the recovery of bio-digestible cellulose from a lignocellulosic byproduct, rice straw, and the increase of ionic liquid utilization. The combined use of ammonia and ionic liquid ([Emim]Ac) treatment exhibited a synergy effect for rice straw with 82% of the cellulose recovery and 97% of the enzymatic glucose conversion. This cooperative effect showed over 90% of the glucose conversion even with a reduced enzyme usage and incubation time. The ionic liquid was successfully recycled more than 20 times. The 20th-recycled ([Emim]Ac) showed 74% of the cellulose recovery and 78 …

Bisphenol A (or BPA) is a toxic endocrine disrupting chemical that is released into the environment through modern manufacturing practices. BPA can disrupt the production, function and activity of endogenous hormones causing irregularity in the hypothalamus-pituitary-gonadal glands and also the pituitary-adrenal function. BPA has immuno-suppression activity and can downregulate T cells and antioxidant genes. The genotoxicity and cytotoxicity of BPA is paramount and therefore, there is an immediate need to properly detect and remediate its influence. In this review, we discuss the toxic effects of BPA on different metabolic systems in the human body, followed by its mechanism of action. Various novel detection techniques (LC-MS, GC-MS, capillary electrophoresis, immunoassay and sensors) involving a pretreatment step (liquid-liquid microextraction and molecularly imprinted solid-phase extraction) have also …

A laboratory study was conducted on the removal of nitrogen and phosphorus from piggery wastewater during growth of Botryococcus braunii UTEX 572, together with measurements of hydrocarbon formation by the alga. The influence was tested of the initial nitrogen and phosphorus concentration on the optimum concentration range for a culture in secondarily treated piggery wastewater. A high cell density (> 7 g L −1 d. wt) was obtained with 510 mg L −1 NO 3 -N. Growth increased with nitrogen concentration at the basal phosphorus concentration (14 mg P L −1 ). The growth rate was nearly independent (μ = 0.027 ∼ 0.030 h −1 ) of the initial phosphate concentration, except under conditions of phosphate deficiency (μ = 0.019 h −1 ). B. braunii grew well in piggery wastewater pretreated by a membrane bioreactor (MBR) with acidogenic fermentation. A dry cell weight of 8.5 mgL −1 and hydrocarbon level of 0.95 gL −1 …

Certain microalgae have been known to use light and various carbon sources to produce carbohydrates, mainly in the form of starch. This is one of the pertinent feedstocks replacing agricultural products for the production of bioethanol by yeast. This study focuses upon dilute acid hydrothermal pretreatments at low cost and high efficiency to compete with current methods, and employs Chlamydomonas reinhardtii UTEX 90 as the feedstock. With dry cells of 5%(w/v), the algal biomass was pretreated with sulfuric acid (1-5%) under temperatures from 100 to , from 15 to 120 min. As a result, the glucose release from the biomass was maximum at 58%(w/w) after pretreatment with 3% sulfuric acid at for 30 min. This method enabled not only starch, but also the hydrolysis of other oligosaccharides in the algal cell in high efficiency. Arrhenius-type of model equation enabled extrapolation of some yields of glucose beyond this range. The pretreated slurry was fermented by yeast, Saccharomyces cerevisiae S288C, resulting in an ethanol yield of 29.2% from algal biomass. This study suggests that the pretreated algal biomass is a suitable feedstock for ethanol production and can have a positive impact on large-scale applied systems.

A synthetic operon for polyhydroxyalkanoate (PHA) biosynthesis designed to yield high levels of PHA synthase activity in vivo was constructed by positioning a genetic fragment encoding β-ketothiolase and acetoacetyl-CoA reductase behind a modified synthase gene containing an Escherichia coif promoter and ribosome binding site. Plasmids containing the synthetic operon and the native Alcaligenes eutro-phus PHA operon were transformed into E. coli DH5α and analyzed for polyhydroxybutyrate production. The molecular weight of polymer isolated from recombinant E. coli containing the modified synthase construct, determined by multiangle light scattering, was lower than that of the polymer from E. coli containing the natjve A. eutrophus operon. A further decrease in polyester molecular weight was observed with increased induction of the PHA biosynthetic genes in the synthetic operon. Comparison of the …

MicroRNAs in exosomes (exosomal miRNAs) have attracted increased attention as cancer biomarkers for early diagnosis and prognosis owing to their stability in body fluids. Since strong association exists between exosomal miRNA expression levels and breast cancer, the development of effective methods that can monitor exosomal miRNA expression both over broad concentration ranges and in ultralow amounts is critical. Here, a surface‐enhanced Raman scattering (SERS)‐based sensing platform is developed for the quantitative determination of exosomal miRNAs. Ultrasensitive exosomal miRNA detection with single‐nucleotide specificity is obtained from enhanced SERS signals from a uniform plasmonic head‐flocked gold nanopillar substrate, which generates multiple hotspots and enables hybridization between short oligonucleotides, i.e., miRNAs and locked nucleic acid probes. The proposed SERS …

Chlamydomonas reinhardtii UTEX 90 accumulated 1.45g dry cell weight and 0.77g starch/L during photosynthetic growth using TAP media at 25 ∘ C in presence of 2% CO 2 for 3 days. C. reinhardtii biomass was concentrated and then converted into hydrogen and organic acids by anaerobic fermentation with Clostridium butyricum. Organic acids in the fermentate of algal biomass were consecutively photo-dissimilated to hydrogen by Rhodobacter sphaeroides KD131. In the concentrated algal biomass 52% of the starch was hydrolyzed to 37.1mmol H 2 /L-concentrated algal biomass and 13.6, 25.5, 7.4 and 493mM of formate, acetate, propionate, and butyrate, respectively by C. butyricum. R. sphaeroides KD131 evolved 5.72mmol H 2 per ml-fermentate of algal biomass under illumination of 8 klux at 30 ∘ C. Only 80% of the organic acids, mainly butyrate, were hydrolyzed during photo-incubation. During anaerobic …

Current research aimed to increase mixotrophic biomass from various organic carbon sources by exploring best light conditions. Three substrates glucose, acetic acid and glycerol were studied for their effects on mixotrophic microalgae cultivation under four light conditions. Light irradiance exhibited variability in growth response and photosynthetic efficiency based on type of substrates used in mixotrophic growth. Each substrate showed variability in light requirements for their effective assimilations. From growth responses, glucose and acetic acid respectively exhibited heterotrophic and mixotrophic (better growth in light) natures. Continuous light-deficient condition was adequate for effective mixotrophic growth as well as energy saving for glucose. However, light-sufficient condition required for effective acetic acid supported mixotrophic growth. Mixotrophic benefits from glycerol and its uptake by Chlorella …

We report a method to build ultrasensitive carbon nanotube-based biosensors using immune binding reaction. Here carbon nanotube–field effect transistors (CNT–FETs) were functionalized with antibody-binding fragments as a receptor, and the binding event of target immunoglobulin G (IgG) onto the fragments was detected by monitoring the gating effect caused by the charges of the target IgG. Because the biosensors were used in buffer solution, it was crucial to use small-size receptors so that the charged target IgG could approach the CNT surface within the Debye length distance to give a large gating effect. The results show that CNT–FET biosensors using whole antibody had very low sensitivity (detection limit ∼1000ng/ml), whereas those based on small Fab fragments could detect 1pg/ml (∼7fM level). Moreover, our Fab-modified CNT–FET could successfully block the nontarget proteins and could …

A biosensor chip utilizing surface plasmon resonance (SPR) was fabricated for detecting anti-glutamic acid decarboxylase (GAD) antibody, which is an indicator of the presence of type I diabetes mellitus. The sensor surfaces were constructed from various thiol mixtures of different molar ratios of 3-mercaptopropionic acid (3-MPA) to 11-mercaptoundecanoic acid (11-MUA). To determine the surface characteristics of the different alkanethiol monolayers, several quantitative and kinetic measurements were carried out. The extent of immobilization of streptavidin (SA) and biotin-GAD (the anti-GAD receptor) and the immune response of anti-GAD antibody against GAD were measured using the SPR biosensor. The terminal functional group of a thiol has different effects on the adsorption and covalent binding of protein depending on the steric hindrance. The protein chip described herein permits simple, rapid detection of …

Algal bioremediation becoming most fascinating to produce biomass as biofuels feedstock while remediating wastes, also improving carbon-footprint through carbon capturing and utilization (CCU) technology. Non-algae process however offers effective treatment but metabolic CO 2 emission is major drawback towards sustainable bioprocess. Mixotrophic cultivation strategy (MCS) enables to treat organic and inorganic wastes which broadly extend microalgae application towards cleaner and sustainable bioeconomy. Latest focus of global think-tanks to encourage bioprocess holding promise of sustainability via CCU ability as important trait. Several high CO 2 emitting industries forced to improve their carbon-footprints. MCS driven microalgae treatment could be best solution for those industries. This review covers recent updates on MCS applications for waste-to-value (biofuels) and environment remediation …

During light induction for astaxanthin formation in Haematococcus pluvialis , we substituted photoautotrophic induction for heterotrophic induction using acetate, both to prevent contamination by heterotrophs due to addition of organic carbon and to enhance carbon assimilation in the induced cells. Strong photoautotrophic induction was performed by N-deprivation of photoautotrophically grown Haematococcus cells followed by supplementation with bicarbonate (HCO 3 − ) or CO 2 . Bicarbonate-induced cells contained more astaxanthin than acetate-induced cells, and even further enhancement of astaxanthin accumulation was achieved by continuous CO 2 supply. The maximum astaxanthin content (77.2 mg g −1 biomass, 3.4-fold higher than with heterotrophic induction) was obtained under conditions of 5% CO 2 , yielding astaxanthin concentration and productivity of 175.7 mg l −1 and 6.25 mg l −1 day −1 , respectively …

Extracellular vesicles (EVs) hold great promise as potential therapeutic carriers. EVs are biologically active, intrinsically transporting cargo between cells. Moreover, they can be loaded with specific cargo for distribution and/or engineered to achieve enhanced uptake. Although studies have already demonstrated therapeutic delivery using EVs, various challenges must be overcome before EV technology is ready for the clinic. Since the properties of EVs are dependent upon their cell of origin and the conditions of their formation, establishing clear characterization practices is essential to ensuring reproducibility and safety. Identifying methods for mass production of EVs is crucial for achieving high EV yields necessary for clinical trials. This review introduces current theory behind EV formation and function, describes the latest methods for characterization and mass production, and discusses future opportunities for …

The uncontrolled, nonspecific adsorption and adhesion of biological materials (eg, proteins and cells) onto synthetic surfaces causes a complex cascade of undesired events1 and bioincompatibility problems2 in the applications of biomaterials. A number of studies on materials, such as polysaccharides, 3 dextran, 4 cellulose dialysis membranes, 5 and poly (ethylene glycol)(PEG), 6 have pursued the elimination/minimization of the unwanted, nonspecific adhesion of biological entities, but the verification of complete rejection to any protein adsorption remains a challenge. PEG shows good biocompatibility, low toxicity, non-immunogenicity, and high water solubility, 7, 8 and in this respect PEG has extensively been investigated as a material with significant inertness to cell and protein adhesion. 9-15 The grafting of PEG-based polymers has been achieved by either the “grafting-onto” 16-18 or the “grafting-from” method. 19, 20 Langer et al. constructed the non-biofouling, PEG-based polymeric surfaces by grafting a PEG-containing, SiO2-reactive polymer onto surfaces. 16 While successful in minimizing the nonspecific adsorption of biological materials, the strictly controlled attachment of biologically active molecules (eg, DNAs, biotin, and peptides), which would have many technologically important applications, such as sensors and microarrays, has not been demonstrated in this approach. The ‘‘graftingfrom” approach, surface-initiated polymerization (SIP), is an alternative to the “grafting-onto” approach. Because the polymer chain is generally grown from surface-anchored polymerization initiators, the lateral and vertical control of the polymer films …

A biochip based on surface plasmon resonance was fabricated to detect prostate specific antigen-α 1 -antichymotrypsin (PSA-ACT complex) in both HBS buffer and human serum. To reduce non-specific binding and steric hindrance effect, the chemical surface of the sensor chips was constructed by using various oligo(ethylene glycol) mixtures of different molar ratios of HS(CH 2 ) 11 (OCH 2 CH 2 ) 6 OCH 2 COOH and HS(CH 2 ) 11 (OCH 2 CH 2 ) 3 OH. The self-assembled monolayers were biotinylated to facilitate the immobilization of streptavidin. Using the chip surfaces, PSA-ACT complex in HBS buffer and human serum was detected at 20.7 and 47.5ng/ml by primary immunoresponse, respectively. However, the limit of detection could be simply enhanced by a sandwich strategy to improve the sensitivity and specificity of the immunoassay. An intact PSA polyclonal antibody was used as an amplifying agent in the strategy. As …

This paper presents a simple and sensitive method for the real-time detection of a prostate cancer marker (PSA-ACT complex) through label-free protein biosensors based on a carbon nanotube field effect transistor (CNT-FET). Herein, the CNT-FET was functionalized with a solution containing various linker-to-spacer ratios, the binding event of the target PSA-ACT complex onto the receptor detected by monitoring the gating effect caused by charges in the target PSA-ACT complex. Since the biosensors were used in a buffer solution, it was crucial to control the distance between the receptors through introduction of linkers and spacers so that the charged target PSA-ACT complex could easily approach the CNT surface within the Debye length to give a large gating effect. The results show that CNT-FET biosensors modified with only linkers could not detect target proteins unless a very high concentration of the PSA …

Professor

나노생명공학연구실

교수님 소개

심상준

연구실 소개

연구실 키워드

알럼나이가 간 회사

연구실 지원 방법

대우 조건

연도별 논문 인용수

저널 & 학회 논문 출판 수

연구실 논문 목록

Peptide-Guided 3D Patterning of Gold Nanoparticles for High-Precision Biosensor Applications

Spectroscopic Analysis of Live Cancer Cell Microenvironment with Terahertz Metasurface Biosensing Platform

Bio-conjugated nanoarchitectonics with dual-labeled nanoparticles for a colorimetric and fluorescent dual-mode serological lateral flow immunoassay sensor in …

연구실 보유 특허

대학별 입시끈

우리 연구실 모집

교수님 소개

심상준

연구실 키워드

알럼나이가 간 회사

연구실 지원 방법

대우 조건

연도별 논문 인용수

저널 & 학회 논문 출판 수

연구실 논문 목록

Peptide-Guided 3D Patterning of Gold Nanoparticles for High-Precision Biosensor Applications

Spectroscopic Analysis of Live Cancer Cell Microenvironment with Terahertz Metasurface Biosensing Platform

Bio-conjugated nanoarchitectonics with dual-labeled nanoparticles for a colorimetric and fluorescent dual-mode serological lateral flow immunoassay sensor in …

연구실 보유 특허

최근 3년간 연구실적 높은 연구실

대학별 입시끈

우리 연구실 모집